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Catalog No.: ML033-100ML
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Denaturation Solution is supplied by HiMedia. It is available in the form of a colourless solution. Denaturation Solution denatures the double-stranded DNA and as a result DNA can be efficiently transferred to nylon or nitrocellulose membrane and subsequently hybridized to a labelled probe during neutral Southern blotting procedure. When denaturation solution is used in conjunction with depurination solution, the phosphodiester backbone at the sites of depurination is hydrolysed which allows a very efficient transfer of large DNA to the membrane. Denaturation Solution is composed of 0.5 M Sodium hydroxide and 1.5 M Sodium chloride. It is advisable to store this product at room temperature (15-25C).
Denaturation Solution is a ready to use buffer solution consists of Sodium chloride and strong base Sodium hydroxide which is especially prepared for use in Southern Blotting and in other in situ hybridization procedures. Denaturation Solution is also used for neutral transfer of double-stranded DNA during Southern blotting procedure.