Hugh Leifson Medium is supplied by the company, HIMEDIA.

This is a Hugh Leifson Medium which serves as a medium for detecting aerobic and anaerobic breakdown of glucose.

Product specifications:

• Catalog number:M826-500G
• Brand: HIMEDIA
• Quantity/unit: Pack of 500g

Product composition:gm/L

• Peptone: 2.000
• Sodium chloride: 5.000
• Dipotassium hydrogen phosphate :0.300
• Dextrose (Glucose): 10.000
• Bromothymol blue: 0.050
• Agar :2.000
• Final pH ( at 25C): 6.80.2

Product features:

• Appearance:Light yellow to bluish green homogeneous free flowing powder
• Gelling: Semisolid,comparable with 0.2% Agar gel.
• Colour and Clarity :Greenish blue coloured, clear to slightly opalescent gel forms in tubes as butts
• Reaction: Reaction of 1.94% w/v aqueous solution at 25C. pH : 6.80.2
• pH: 6.60-7.00
• Cultural Response: Cultural characteristics observed after an incubation at 35-37C for 18-48 hours