ISP Medium No. 4 also cited as Inorganic Salt Starch Agar, is supplied by the company, HIMEDIA.

ISP Medium No. 4serves as a medium for cultivation and characterization ofStreptomycesspecies as per InternationalStreptomycesProject

Product specifications:

• Catalog number:M359-500G
• Brand: HIMEDIA
• Quantity/unit: Pack of 500g

Product composition:gm/L

• Starch, soluble: 10.000
• Dipotassium hydrogen phosphate: 1.000
• Magnesium sulphate heptahydrate :1.000
• Sodium chloride :1.000
• Ammonium sulphate :2.000
• Calcium carbonate :2.000
• Ferrous sulphate heptahydrate: 0.001
• Manganous chloride, heptahydrate: 0.001
• Zinc sulphate heptahydrate :0.001
• Agar: 20.000
• Final pH ( at 25C): 7.20.2

Product features:

• Appearance: Cream to yellow homogeneous free flowing powder
• Gelling :Firm, comparable with 2.0% agar gel
• Colour and Clarity of prepared medium:Light amber coloured, opalescent gel forms in Petri plates
• Reaction: Reaction of 3.65% w/v aqueous solution at 25C. pH : 7.20.2
• pH :7.00-7.40
• Cultural Response: Cultural characteristics observed after an incubation at 30-32C for 48-72 hours