High grade KF Streptococcal Agar Base is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder which has a pH in the range of 7.0 to 7.4. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 100g /pack

KF Streptococcal Agar Base is mainly used for selective isolation and enumeration of faecal Streptococci in surface water by direct plating or by membrane filter method. Streptococci are spherical, gram-positive bacteria and a part of the normal flora of the mouth, skin, intestine, upper respiratory tract of humans. Faecal Streptococci are found in the faeces and constitute of group D Lancefield antigens. The types include Streptococcus faecalis, Streptococcus faecium, Streptococcus bovis and Streptococcus duran. They are low-grade pathogens and rarely cause disease. Kenner-Faecal (KF) Medium was developed by Kenner et al (1, 2) for detecting Streptococci in water and food materials. Special peptone with yeast extract provide nitrogen, carbon, sulphur, amino acids, vitamins and trace ingredients to the faecal Streptococci. Lactose and maltose are the fermentable carbohydrates and therefore serve as energy sources. Sodium azide is a selective agent, which hampers the growth of gram-negative bacteria. Bacteria resistant to azide, utilize lactose and /or maltose. 2,3,5-Triphenyl Tetrazolium Chloride is reduced to insoluble formazan by actively metabolizing cells, resulting in the formation of pink or red colonies. The acidity so produced changes the colour of the indicator dyes to yellow. Bacterial cells reduce TTC to insoluble formazan, resulting in the formation of pink to red colonies.