M-Endo Broth is supplied by Micromaster. It is available in the form of a light pink to purple homogeneous free flowing powder which has a pH in the range of 7.3 to 7.7. It is advisable to store this product below 30C.

Quantity provided: 500 g/pack

M-Endo Broth is used for estimation of coliforms in water samples using membrane filter technique. It is possible to remove bacteria from fluids by passing them through filters with such small pore size that bacteria are arrested. This filtration technique enables fairly large volumes of water to pass rapidly under pressure, but prevents the passage of any bacteria present. These nutrients are retained on the surface of the membrane which is then brought into contact with suitable liquid nutrients. M-Endo Broth was used for studying milk lines of milk handling equipment and for examination of swimming pool waters using membrane filter technique. This medium gives higher counts and is most satisfactory of the many media used, since coliform colonies develop rapidly, preliminary enrichment and saturated relative humidity are not necessary and results are in good agreement with the Standard Methods MPN Test. Peptic digest of animal tissue and yeast extract present in this broth, provide essential nutrients especially nitrogenous for the coliforms. Lactose is the fermentable carbohydrate. Sodium sulphite and basic fuchsin inhibit the growth of gram-positive organisms. Phosphates buffer the medium. Coliforms ferment lactose and the resulting acetaldehyde reacts with sodium sulphite and basic fuchsin to form red colonies and similar colouration of the medium. Lactose non-fermenters form colourless colonies.