High grade Mueller Kauffman Tetrathionate Broth Base is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder. For maximum efficiency, it must be stored below 30C in tightly closed container. Mueller Kauffman Tetrathionate Broth Base conforms to ISO specifications.

Quantity provided: 500g /pack

Mueller Kauffman Tetrathionate Broth is used for improved enrichment and isolation of Salmonellae since the examination of various types of food products for Salmonella requires different methods. This is because of the low numbers of Salmonellae in foods and the frequently poor physiological state of these pathogens following exposure to stressful conditions during food processing or storage. The ideal pre-enrichment broth should provide for the repair of cell damage, dilute toxic or inhibitory substances and nutritive enough to favour growth of Salmonella. Selective enrichment media contains selective ingredients that allow the proliferation of Salmonella and inhibit the growth of competing non-salmonella microorganisms. For the detection of foodborne Salmonella, or to identify Salmonella from animal feces and sewage water, Mueller recommended Tetrathionate Broth as a selective medium. Kauffman modified the formula to include ox bile and brilliant green as selective agents to suppress bacteria like Proteus species. Selectivity is conferred by tetrathionate (from the reaction of thiosulphate and iodine). This media contains casein enzymic hydrolysate and papaic digest of soyabean meal as sources of carbon, nitrogen, vitamins and minerals. Ox bile and added brilliant green are selective agents, which inhibit gram-positive and other gram-negative organisms. Calcium carbonate is the buffer. Sodium chloride maintains osmotic equilibrium. Sodium thiosulphate is a source of sulfur. The tetrathionate (S4O6) anions constitute the principle selective agent in these enrichment media.