High grade Reinforced Medium for Clostridia is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder with a pH in the range of 6.6 to 7.0. It is advisable to store this product below 30C.

• Quantity provided: 100 g/pack

Reinforced Medium for Clostridia was formulated by Hirsch and Grinsted. This media is prepared in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP. It is recommended for checking the sterility of non-sterile products, nutritional and dietary supplements. It can be used to initiate growth from small inoculum and to obtain the highest viable count of clostridia. It can also be used in studies of spore forming anaerobes, especially Clostridium butyricum in cheese, for enumeration of Clostridia in tube dilution counts or for preparation of plates for isolation. Other spore forming anaerobes, Streptococci and Lactobacilli also grow in these media. These are enriched but non-selective media. Peptone, yeast extract and beef extract provide all the necessary nutrients for the growth of clostridia. Glucose monohydrate is a fermentable carbohydrate in the medium while sodium chloride maintains osmotic equilibrium. Cysteine hydrochloride acts as reducing agent. Small amount of soluble starch removes toxic metabolites from the medium. Sodium acetate also acts as a good buffering agent. Small quantity of agar keeps the medium semi solid and helps in maintaining anaerobic conditions.