High grade Urea Broth Base is supplied by Micromaster. It is available in the form of a light yellow to light pink homogenous free flowing powder with a pH in the range of 6.6 to 7.0. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

• Quantity provided: 100 g/pack

Urea Broth is recommended for the identification of bacteria on the basis of urea utilization, specifically for the differentiation of Proteus species from Salmonella and Shigella species. Rustigian and Stuart developed Urea Broth. This medium is especially recommended for the differentiation of Proteus species from Salmonella and Shigella species in the enteric infection diagnosis, based on urea utilization. Gram-negative enteric bacilli are unable to utilize urea because of less nutrients and high buffering capacity of the medium. Urea Broth becomes alkaline as the utilization of urea by the organisms liberates ammonia during the incubation, indicated by pink red colour. All urea test media rely on the alkalinity formation and so they are not specific for urease testing. The utilization of proteins may raise the pH to alkalinity due to protein hydrolysis and excess of amino acids results in false-positive reaction. A medium without urea serves as negative control to rule out false positive results.