High grade Violet Red Bile Glucose Agar is supplied by Micromaster. It is available in the form of a light yellow to pinkish beige homogenous free flowing powder which has a pH in the range of 7.2 to 7.6. It is advisable to store this product below 30C.

• Quantity provided: 500 g/pack

Violet Red Bile Glucose Agar is a selective medium recommended for detection and enumeration of Enterobacteriaceae from pharmaceutical products using the microbial limit testing in compliance with the harmonized methodology of USP/EP/BP/JP. Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to presence of the inhibitors; bile salts and crystal violet. Crystal violet inhibits gram-positive organisms especially Staphylococci. Neutral red indicator helps to detect lactose and glucose fermentation. Glucose fermenting strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium. The red colour is due to absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8. The sample is initially enriched in Enterobacteria Enrichment broth -Mossel and then subcultured on Violet Red Bile Glucose Agar.