0.3M Tris Buffer
₹ 3,237.00 *
Catalog No.: ML126-500ML
Usually Shipped in: 2-3 Weeks
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0.3M Tris Buffer is supplied by HiMedia. It is available in the form of a colourless solution with a pH in the range of 10.1 to 11.1. Tris is the common name of tris (hydroxymethyl) aminomethane. It is a primary amine and is effective as a buffer in the pH range. Tris does not precipitate calcium salts and maintains solubility of manganese salts. It has a low UV absorbance but strong temperature sensitivity. This buffer is supplied as 0.3M. It is advisable to store this product at room temperature (15-25°C). This solution is ready for use and does not require any extra preparation.
Tris Buffer is used extensively in Molecular Biology. It is also used as the major component of several buffer solutions, e.g. Tris-EDTA buffer, Tris-acetate EDTA buffer, Tris-borate EDTA buffer, Tris-buffered saline etc. Tris HCl is an organic compound which is highly soluble in water. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification. This product can be diluted to obtain the desired concentration.