10X TBST is supplied by HiMedia. It is a 10X concentrated solution of Tris Buffered Saline with Tween 20. It contains 100mM Tris.HCl, 150mM NaCl, 0.5% Tween 20 at pH 7.5. Dilute Tris Buffered Saline with Tween 20 (TBST-10X) to a 1X solution using ddH2O.

TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.