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Catalog No.: ML007-500ML
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20% SDS is supplied by HiMedia. It is available in the form of a colourless solution. 20% SDS solution is a standard reagent extensively used in molecular biology. This solution is provided in a convenient ready to use format that overcomes the requirement of handling SDS powder which is an irritant. This solution can be used for preparing buffers which has several applications including SDSPAGE, blot hybridization, various washing protocols etc. It is advisable to store this product at room temperature (15-25C).
Sodium dodecyl sulphate is an anionic surfactant that is extensively used in biochemistry and molecular biology. Each SDS molecule has a tail of 12 carbon atoms which are attached to a sulphate group. This provides SDS an amphiphilic property which is required for a detergent. This multipurpose, anionic detergent can be used for protein solubilization, plasmid extraction from bacteria, and to reduce non-specific binding during nucleic acid hybridization on membranes. SDS is the most commonly used detergent in protein purification and electrophoresis. It is used as a protein denaturing agent during both extraction procedures and SDS polyacrylamide gel electrophoresis. Negatively charged anionic detergents are strong detergents utilized in lysis buffers for the complete disruption of membranes and denaturation of proteins. SDS disrupts the non-covalent bonds of the proteins and denatures them.