CHU (N6) Macroelements is supplied by HiMedia. It is available in the form of a white to off-white, homogenous, free flowing powder with a pH in the range of 4.2 to 5.2. Since this product is extremely hygroscopic, it is advisable to store it at 2-8C, in air tight containers. The composition (mg/ml) is as follows:

Ammonium sulphate (463.00)

Calcium chloride (125.34)

Magnesium sulphate (90.370)

Potassium nitrate (2830.00)

Potassium phosphate monobasic (400.00)

CHU (N6) Medium has been developed by C.C. et al. in 1975 for the in vitro anther culture of oryza sativa family Graminae. The medium helps in the initiation, growth and differentiation of callus from the rice pollen cultures. CHU (N6) Macroelements is a nutrient blend of inorganic salts that provides all essential macroelements to the plants. Potassium nitrate serves as a source of nitrate. Ammonium sulphate quantity has been reduced as ammonium ions have inhibitory effect on growth and quality of the rice callus but aids in morphogenesis. Magnesium sulphate provides magnesium which acts as a precursor in many vital metabolic processes. Calcium serves as a modulator and helps in cell wall synthesis and stabilization.