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EXTRACTME RNA & DNA KIT, 50 preps

EXTRACTME RNA & DNA KIT, 50 preps

₹ 36,663.90 *
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  • Brand: Blirt
  • Catalog No.: EM15-050
  • Quantity/Unit: 50 preps/pack

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EXTRACTME RNA & DNA KIT, 50 preps supplied by BLIRT is intended to ensure a quick and effective isolation of high-quality total RNA and genomic DNA from a single biological sample simultaneously. The protocol of isolation and buffer formulations was optimized to ensure high efficiency of isolation and purity of RNA and DNA. The process for RNA purification uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively at high concentration of chaotropic salts. The 1st isolation step involves the homogenization of a tissue which results in the disintegration of intercellular bonds (epithelial tissue) and fragmentation of high-molecular proteins (muscle or connective tissue). Lysing of the homogenate is done with guanidine thiocyanate and detergents. Guanidine thiocyanate and -mercaptoethanol (optional) is utilized to inactivate Nucleases. The separation of the homogenate from undigested tissue/cell that remains after centrifugation is done. The DNA then binds to the DNA Purification Column membrane and RNA remaining in the filtrate binds to the RNA Purification Column membrane by selective conditions in mixture after adding ethanol. A three-step washing stage adequately eliminates contaminations and protein inhibitors. By the help of low ionic strength buffer the purified DNA & RNA is eluted out that may be utilized in several downstream applications, like PCR, qPCR, primer extension, sequencing, microarrays, Southern blotting, and in case of RNA also Northern blotting, RT-PCR, cDNA synthesis or stored until ready to use.

Product Specifications:

Product name - EXTRACTME RNA & DNA KIT, 50 preps

Catalog No - EM15-050

Brand: BLIRT

Pack size: 50 preps

Product Features:

1 SAMPLE MATERIAL

fresh or frozen tissue: up to 30 mg

tissue preserved in RNase inactivating buffers (e.g. RNAlater , Ambion):

up to 30 mg

cell culture: up to 107 cells

2. BINDING CAPACITY

~230 g RNA, ~60 g DNA

3. TIME REQUIRED

10 minutes (for simultaneous RNA/DNA purification, lysis and homogenization time not included)

1530 minutes for homogenization in liquid nitrogen

1520 minutes for mechanical homogenization (ceramic beads)

4. RNA/DNA PURITY

A260/A280 ratio = 1.9 2.1

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