HiPurA 96 Yeast Genomic DNA Purification Kit is supplied by HiMedia. HiPurA Genomic DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, Southern blotting technique etc. HiMedias 96-well format allows rapid processing of multiple samples. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR amplification and Southern blotting. This kit contains:

Lysis Solution (AL)

Lysis Solution (C1)

Prewash Solution (PWB)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiPurA 96-well Block (1.6 ml)

HiPurA 96-well DNA Plate

HiPurA Silicon Pad for sealing

HiPurA 96-well V-plate

HiPurA 96-well Block (2.2 ml)

This kit simplifies isolation of DNA from yeast by a simple procedure. Yeast cells (Saccharomyces cerevisiae, Candida albicans) are grown in log phase and spheroplasts are subsequently prepared. Following lysis, the DNA is bound to the silica-gel membrane of the HiPurA 96-well DNA plate (DBPL-96-01). The rapid wash step remove trace amount of salt and protein contaminants resulting in the elution of pure DNA in the Elution Buffer provided with the kit.