HiPurA Plant and Fungal RNA Miniprep Purification Kit is supplied by HiMedia. HiPurA Plant and Fungal RNA Miniprep Purification Kit provides a fast and easy method for purification of total RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT-PCR, Differential display, Expression-array, expression-chip analysis and cDNA library construction. The RNA purification procedure using the miniprep spin columns comprises of three steps namely, RNA getting absorbed to the membrane, removal of residual contaminants and elution of pure RNA. HiElute Miniprep Spin Column (Capped) format enables rapid processing of multiple samples. These columns have a high binding capacity and high quality RNA is obtained from various species. The RNA obtained is compatible with various downstream applications as mentioned above. This kit contains:

RNA - XPressTM Reagent

Binding Solution (PBR)

Prewash Solution (RW1)

Wash Solution Concentrate (WS)

Elution Solution (RNase- Free Water)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit makes isolation of RNA from plant cells, tissues and filamentous fungi easier with spin column procedure. The RNA - XPress Reagent provided in the kit aids in cell disruption and denaturation of the plant material. After the addition of chloroform and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing cell debris and DNA and an organic phase containing proteins. Binding Solution (PBR) and ethanol are added to the aqueous phase, which promotes selective binding of RNA to the HiElute Miniprep Spin Column (Capped) membrane. After the initial binding of RNA, short washing steps are used to remove impurities like proteins, polysaccharides, low molecular weight metabolites and salts. High quality RNA is finally eluted in the Elution Solution provided with the kit. Typical yield is around 15-65 g of total RNA from 100 mg of the plant material, depending on the type of tissue and plant species.