TRI Reagent

TRI reagentis a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagentis a mixture of guanidine thiocyanate and phenol in a monophasic solution.

Homogenization or lysis of the tissue sample in TRI reagentdissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.

Application

TRI Reagent has been used:

• In the isolation of total RNA from various tissue samples and cells
• For the lysis of monocytes
• To isolate cytoplasmic RNA from SARS-CoV infected Vero cells
• To isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
• To prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine