DNA Purification and Extraction
Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
GeneiPure Mammalian Genomic DNA Purification Kit-2115500031730
₹ 16,570.00
- Brand:
Bangalore GeNei - Catalog No.:
2115500031730 - Quantity/Unit: 50 preps/Kit
- Availability: In stock
UniFlex DNA Isolation Kit-2117000051730
₹ 14,120.00
- Brand:
Bangalore GeNei - Catalog No.:
2117000051730 - Quantity/Unit: 100 preps/Kit
- Availability: In stock
Bacterial DNA Extraction Kit, 50 Extractions
₹ 6,634.00
- Brand:
TEKBIO - Catalog No.:
TBAC-50 - Quantity/Unit: 50 Extractions/Box
- Usually Shipped in: 1-2 Weeks
Insect DNA Extraction Kit, 50 Extractions
₹ 6,634.00
- Brand:
TEKBIO - Catalog No.:
TIDK-50 - Quantity/Unit: 50 Extractions/Box
- Usually Shipped in: 1-2 Weeks
Milk DNA Extraction Kit, 50 Extractions
₹ 7,438.00
- Brand:
TEKBIO - Catalog No.:
TMDK-50 - Quantity/Unit: 50 Extractions/Box
- Usually Shipped in: 1-2 Weeks
Plasmid DNA Isolation Kit- MPK2
₹ 5,900.00
- Brand:
Aura Biotech - Catalog No.:
ABT-171M - Quantity/Unit: 50 Rxns/Pack
- Usually Shipped in: 4-7 Days
GeNei Whole Blood DNA Extraction Kit -2102300011730
₹ 7,590.00
- Brand:
Bangalore GeNei - Catalog No.:
2102300011730 - Quantity/Unit: 50 preps/Kit
- Availability: In stock
GeNei Gel Extraction Kit-2100200051730
₹ 5,130.00
- Brand:
Bangalore GeNei - Catalog No.:
2100200051730 - Quantity/Unit: 100 preps/Kit
- Availability: In stock
GeNei Plasmid DNA Purification Teaching Kit-6112600011730
₹ 5,870.00
- Brand:
Bangalore GeNei - Catalog No.:
6112600011730 - Quantity/Unit: 10 expts./Kit
- Availability: In stock
GeNei Genomic DNA Extraction Teaching Kit-6102800011730
₹ 6,140.00
- Brand:
Bangalore GeNei - Catalog No.:
6102800011730 - Quantity/Unit: 10 expts/Kit
- Availability: In stock
DNA Extraction Kit-Plant-TPDK-50
₹ 5,655.00
- Brand:
Transiom Genomics - Catalog No.:
TPDK-50 - Quantity/Unit: 50 Extraction/Box
- Usually Shipped in: 4-7 Days
Gel DNA/PCR Purification Kit-TGPK-K50
₹ 4,875.00
- Brand:
Transiom Genomics - Catalog No.:
TGPK-K50 - Quantity/Unit: 50 Purification/Box
- Usually Shipped in: 4-7 Days
DNA Extraction Kit-Tissue-TTDK-50
₹ 7,410.00
- Brand:
Transiom Genomics - Catalog No.:
TTDK-50 - Quantity/Unit: 50 Extraction/Box
- Usually Shipped in: 4-7 Days
DNA Extraction Kit-Fungal-TFDK-50
₹ 6,825.00
- Brand:
Transiom Genomics - Catalog No.:
TFDK-50 - Quantity/Unit: 50 Extraction/Box
- Usually Shipped in: 4-7 Days
DNA Spin Column-TNSC-50-DNA
₹ 30.00
- Brand:
Transiom Genomics - Catalog No.:
TNSC-50-DNA - Quantity/Unit: 1 pc/Pack
- Usually Shipped in: 4-7 Days
HiPer DNA Estimation Teaching Kit-HTBC006-20PR
₹ 9,755.00
- Brand:
HIMEDIA - Catalog No.:
HTBC006-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer DNA Estimation Teaching Kit-HTBC006-5PR
₹ 3,170.00
- Brand:
HIMEDIA - Catalog No.:
HTBC006-5PR - Quantity/Unit: 5PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Plasmid DNA Extraction Teaching Kit-HTBM002-10PR
₹ 3,480.00
- Brand:
HIMEDIA - Catalog No.:
HTBM002-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Plasmid DNA Extraction Teaching Kit-HTBM002-20PR
₹ 6,440.00
- Brand:
HIMEDIA - Catalog No.:
HTBM002-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Plasmid DNA Extraction Teaching Kit-HTBM003-20PR
₹ 5,880.00
- Brand:
HIMEDIA - Catalog No.:
HTBM003-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Plant Genomic DNA Extraction Teaching Kit-HTBM004-10PR
₹ 4,375.00
- Brand:
HIMEDIA - Catalog No.:
HTBM004-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Plant Genomic DNA Extraction Teaching Kit-HTBM005-10PR
₹ 4,065.00
- Brand:
HIMEDIA - Catalog No.:
HTBM005-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Blood Genomic DNA Extraction Teaching Kit-HTBM006-10PR
₹ 3,480.00
- Brand:
HIMEDIA - Catalog No.:
HTBM006-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Blood Genomic DNA Extraction Teaching Kit-HTBM006-20PR
₹ 6,440.00
- Brand:
HIMEDIA - Catalog No.:
HTBM006-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Blood Genomic DNA Extraction Teaching Kit-HTBM007-10PR
₹ 3,315.00
- Brand:
HIMEDIA - Catalog No.:
HTBM007-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Blood Genomic DNA Extraction Teaching Kit-HTBM007-20PR
₹ 6,135.00
- Brand:
HIMEDIA - Catalog No.:
HTBM007-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Bacterial Genomic DNA Extraction Teaching Kit-HTBM008-10PR
₹ 2,950.00
- Brand:
HIMEDIA - Catalog No.:
HTBM008-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks
HiPer Bacterial Genomic DNA Extraction Teaching Kit-HTBM009-10PR
₹ 3,380.00
- Brand:
HIMEDIA - Catalog No.:
HTBM009-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 2-3 Weeks