GenProTMDNA/RNA/Protein isolation kit provides a rapid and easy method for the simultaneous extraction of total RNA, genomic DNA and proteins from a single sample using spin column technology. The total RNA, genomic DNA and protein are purified in less than 1 hour. This quick, reliable and consistent technique provides a simple and effective way to study protein and nucleic acids simultaneously from bacteria and yeast, plants, cells and tissues, blood and other biological samples without affecting yield and quality. This isolation kit is suitable for the isolating of macromolecules from the precious samples without fractionating the samples. The obtained DNA, RNA and protein have been tested for relevant downstream applications without or minimal cross contamination.