High grade Lauryl Sulphate Broth (Lauryl Tryptose Broth) is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder which has a pH in the range of 6.6 to 7.0. It is advisable to store this product between 15-25C.

• Quantity provided: 100 g/pack

Lauryl Sulphate Broth (Lauryl Tryptose Broth) is used for the detection of coliforms in water, wastewater, dairy products and other food samples. Coliforms are considered to be members of Enterobacteriaceae, which grow in the presence of bile salts and produce acid and gas from lactose within 48 hours at 37C. Lauryl Sulphate Broth is designed to obtain rich growth and substantial amount of gas from small inocula of coliform organisms. Aerobic spore-bearers are completely inhibited in this medium. Tryptose provides essential growth substances, such as nitrogen and carbon compounds, sulphate and trace ingredients. The potassium phosphates provide buffering system, while sodium chloride maintains osmotic equilibrium. Sodium lauryl sulphate inhibits organisms other than coliforms.For inoculum of 1 ml or less, use single strength medium. For inocula of 10 ml or more, double strength or proportionate medium should be prepared. After inoculation, incubate the tubes at 37C for 24 to 48 hours. For every tube showing fermentation (primary fermentation), inoculate two tubes of Lauryl Tryptose Broth from the tube showing primary fermentation and incubate these tubes at 37C and 44C respectively.