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HiPurA Food DNA Purification Kit

HiPurA Food DNA Purification Kit

₹ 35,785.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MB562-250PR
  • Quantity/Unit: 250pr*/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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HiPurA Food DNA Purification Kit is supplied by HiMedia. HiPurA Food DNA Purification Kit provides a fast and easy method for purification of total DNA from various sources of food samples for downstream applications such as PCR, RT-PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin column comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality genomic DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR and Southern blotting. This kit contains:

Lysis Solution (C1)

Binding solution (FB)

Prewash Solution Concentrate (PW)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiEluteTM Miniprep Spin Column (in PW1139 Collection Tube)

Collection Tubes, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from variety of raw and processed food matrices. Genomic DNA purification from food involves cell lysis, which is achieved by incubation of food in a solution containing chaotropic ions in the presence of Proteinase K at 65C. Following lysis, DNA binds to the silica gel membrane of the HiElute Miniprep Spin Column to yield purified DNA. After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit.

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