HiPurA Water DNA Purification Kit is supplied by HiMedia. HiPurA Water DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz, adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR amplification and Southern blotting. This kit contains:

Gram Positive Lysis Solution (GPLA)

Lysis Solution (AL)

Lysis Solution (C1)

Prewash Solution (PWB)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

Lysozyme

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

HiPurA Water DNA Purification Kit provides a convenient and rapid method for detection of microorganisms from environmental water samples. The kit allows rapid isolation and purification of total DNA from bacteria and fungi found in water samples. The water sample is passed through a 0.22 filter and the microorganisms present in the water are trapped. Cells are grown in the medium till they reach log phase and are harvested by centrifugation. After harvesting, the bacterial (Gram positive) cell wall is degraded by lysozyme and Proteinase K. For Gram negative bacteria, the lysozyme treatment is not required. Following lysis, the DNA is bound to the silica-gel membrane of the HiElute Miniprep Spin Column (Capped) to yield pure DNA. Two rapid wash steps remove trace amount of salt and protein contaminants resulting in the elution of pure DNA in the Elution Buffer provided with the kit. The rigorous treatment with Proteinase K disrupts the cell wall and proteolytic digestion of the cellular protein occurs. Furthermore, Proteinase K completely denatures the histones and histone-like proteins. Hence, depending upon the downstream applications one can isolate genomic DNA from yeast either by using Proteinase K (following alternative protocol) or Lyticase.