Hugh Leifson Medium is supplied by the company, HIMEDIA.

Hugh Leifson Medium serves as a medium to distinguish between anaerobic and aerobic breakdown of carbohydrate (glucose).

Product specifications:

• Catalog number:M826S-100G
• Brand: HIMEDIA
• Quantity/unit: Pack of 100g

Product composition:gm/L

• Peptone: 2.000
• Sodium chloride :5.000
• Dipotassium phosphate :0.300
• Glucose (Dextrose): 10.000
• Bromothymol blue: 0.030
• Agar: 3.000
• Final pH ( at 25C): 7.10.2

Product features:

• Appearance: Light yellow to bluish green homogeneous free flowing powder
• Gelling: Semisolid,comparable with 0.2% Agar gel
• Colour and Clarity: Greenish blue coloured, clear to slightly opalescent gel forms in tubes as butts
• Reaction: Reaction of 2.03% w/v aqueous solution at 25C. pH : 7.10.2
• pH :6.90-7.30
• Cultural Response: Cultural characteristics observed after an incubation at 35-37C for 18-48 hours