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Catalog No.: ML017-100ML
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50X TBE, is supplied by HiMedia. It is available in the form of solid tablets. These 50X TBE - Concentrate Gel Tablets come in easy-to-open pouches for simple and quick preparation of TBE Buffer. 1000 ml of 1X buffer can be prepared from each 20ml tablet. It is advisable to store this product room temperature (15-25C). For preparing 1X buffer, you can add the tablet in 950 ml of Molecular Biology Grade Water/ distilled water and stir the mixture, till it dissolved. Then make up the volume to 1 litre using Molecular Biology Grade Water /distilled water.
TBE (Tris-Borate-EDTA) buffer is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments; whereas, TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. A solid, tablet form of the product allows for the convenient, space-saving storage of TBE in a non-precipitable form. The 50X TBE - Concentrate Gel Tablets come individually pouched and require heating for less than a minute in a microwave in water to dissolve. The dissolved tablet is then reconstituted to the appropriate volume to yield the required TBE solution. Fresh TBE can be made in a matter of minutes for any electrophoresis application. The tablet format allows for a longer shelf life without precipitation problems and also decreases the storage space normally required for liquid TBE.