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HiPurA Buccal DNA Purification Kit

HiPurA Buccal DNA Purification Kit

₹ 39,195.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MB531-250PR
  • Quantity/Unit: 250pr*/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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HiPurA Buccal DNA Purification Kit is supplied by HiMedia. HiPurA Buccal DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR and Southern blotting technique, etc. The DNA purification procedure using the miniprep spin column comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR and Southern blotting. This kit contains:

Resuspension Solution (1X PBS)

Lysis Solution (C1)

Prewash Solution Concentrate (PW)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tubes (Uncapped), Polypropylene (2.0 ml)

Collection Tubes, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from the inside of each cheek or gargled samples with spin column procedure. Following lysis, DNA binds to silica gel membrane of the HiElute Miniprep Spin Column (Capped) to yield purified of DNA. Two rapid wash steps remove trace salts and protein contaminants resulting in the elution of high quality DNA in the Elution Buffer (ET) provided with the kit. Typical yield of DNA is 0.5-3.5 g of DNA.

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