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MEM (Joklik Modification) (For Suspension Culture) w/ L-Glutamine w/o CaCl, NaHCO3

MEM (Joklik Modification) (For Suspension Culture) w/ L-Glutamine w/o CaCl, NaHCO3

₹ 4,255.00 *
  • Brand: HIMEDIA
  • Catalog No.: AT079-5L
  • Quantity/Unit: 5lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack

For lab/research use only, unless otherwise specified


Minimum Essential Medium Eagle (MEM) Joklik modification (For Suspension Culture) With L-Glutamine Without Calcium chloride and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.8 -5.4 whereas the pH with Sodium Bicarbonate is between 6.5 -7.1. The osmolality without Sodium Bicarbonate is in the range of 250.00 -290.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 290.00 -330.00 Osm/Kg H20. Media composition (in mg/L) is as follows:

Magnesium chloride (93.68)

Potassium chloride (400.00)

Sodium chloride (6500.00)

Sodium dihydrogen phosphate (1154.00)

Amino acids

Choline chloride (1.00)

D-Ca-Pantothenate (1.00)

Folic acid (1.00)

Nicotinamide (1.00)

Pyridoxal hydrochloride (1.00)

Riboflavin (0.10)

Thiamine hydrochloride (1.00)

i-Inositol (2.00)

D-Glucose (2000.00)

Phenol red sodium salt (11.00)

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. This product is the Joklik modification of Minimum Essential Medium. As it is designed for use with suspension cells, it does not contain calcium.

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