PCR
Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.
In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.
GenoDTech MERS Real Time PCR Kit, 100 Reactions
- Brand:
GenoDTech - Catalog No.:
TLPL-016 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Covid-19 Real Time PCR Kit, 100 Reactions
- Brand:
GenoDTech - Catalog No.:
TLPL-017 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Covid19 + MERS Real Time PCR Kit, 100 Reactions
- Brand:
GenoDTech - Catalog No.:
TLPL-018 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Influenza Real Time PCR Kit, 100 Reactions
- Brand:
GenoDTech - Catalog No.:
TLPL-020 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Dengue Real Time PCR Kit
- Brand:
GenoDTech - Catalog No.:
TLPL-021 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Malaria Real Time PCR Kit
- Brand:
GenoDTech - Catalog No.:
TLPL-022 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Varicella-Zoster virus (VZV) Real Time PCR Kit
- Brand:
GenoDTech - Catalog No.:
TLPL-023 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Chikungunya Real Time PCR Kit
- Brand:
GenoDTech - Catalog No.:
TLPL-024 - Quantity/Unit: 100 Reactions/Pack
GenoDTech Covid19 + MERS + Influenza Real Time PCR Kit, 100 Reactions
- Brand:
GenoDTech - Catalog No.:
TLPL-028 - Quantity/Unit: 100 Reactions/Pack
Optical Plate Seal- 157300
- Brand:
TARSONS - Catalog No.:
157300 - Quantity/Unit: 100/Pack
- Usually Shipped in: 1-2 Weeks
Thermo Conductive Rack for 1.5ml tubes and PCR tubes or stripes
- Brand:
TARSONS - Catalog No.:
525340 - Quantity/Unit: 1No/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp PCR Tubes-510053
- Brand:
TARSONS - Catalog No.:
510053 - Quantity/Unit: 1000/Pack
- Usually Shipped in: 1-2 Weeks
Deep Well Storage Plates - 0.8 ml-510066
- Brand:
TARSONS - Catalog No.:
510066 - Quantity/Unit: 50/Pack
- Usually Shipped in: 1-2 Weeks
Deep Well Storage Plates -1.2 ml -510067
- Brand:
TARSONS - Catalog No.:
510067 - Quantity/Unit: 50/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with Cap -510073
- Brand:
TARSONS - Catalog No.:
510073 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with Cap -610040
- Brand:
TARSONS - Catalog No.:
610040 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with Cap -610050
- Brand:
TARSONS - Catalog No.:
610050 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with Cap -610060
- Brand:
TARSONS - Catalog No.:
610060 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2 ml clear tube strips with cap
- Brand:
TARSONS - Catalog No.:
610070 - Quantity/Unit: 125Nos/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with attached Cap -610080
- Brand:
TARSONS - Catalog No.:
610080 - Quantity/Unit: 120/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with attached Cap -610090
- Brand:
TARSONS - Catalog No.:
610090 - Quantity/Unit: 120/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.1ml Low Profile Tube Strips with Cap-611020
- Brand:
TARSONS - Catalog No.:
611020 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.1ml Low Profile Tube Strips with Cap-611030
- Brand:
TARSONS - Catalog No.:
611030 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
Maxiamp 0.1ml Low Profile Tube Strips with Cap-611040
- Brand:
TARSONS - Catalog No.:
611040 - Quantity/Unit: 125/Pack
- Usually Shipped in: 1-2 Weeks
First Stranded cDNA Synthesis Kit (AMV), 25 Rxn
- Brand:
APS LABS - Catalog No.:
MAG01A - Quantity/Unit: 25 Rxn/Vial
- Usually Shipped in: 4-7 Days
MAGscript First Stranded cDNA Synthesis, 100 Rxn
- Brand:
APS LABS - Catalog No.:
MAG01-II-PLUS - Quantity/Unit: 100 Rxn/Vial
- Usually Shipped in: 4-7 Days
Lambda DNA 0.1 mg, 0.1mg/mL
- Brand:
APS LABS - Catalog No.:
APSN-19 - Quantity/Unit: 0.1mg/mL/Vial
- Usually Shipped in: 4-7 Days
LBC/ HPV DNA Sample Collection kit, 1 Kit
- Brand:
AROMICS - Catalog No.:
D1550-1 - Quantity/Unit: 1 Kit/Pack
- Availability: In stock