Denhardt's Solution (100X), is supplied by HiMedia. Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin. Sterile filtered. It is advisable to store this product between -5 to -30C.

Denhardt's Solution is used as a blocking reagent for northern or southern blots to prevent probe binding on open membrane space and non-target nucleic acids. Denhardt's reagent is recommended for Northern hybridization, hybridization of RNA, single copy Southern hybridization' or hybridization of DNA, immobilized on nylon membranes.