Synta-benzoNuk benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and circular) to form oligodeoxynucleotides with terminal 5'-monophosphates of 3-5 bases. Effective over a wide range of operating conditions. Has no proteolytic activity.Isolated from Escherichia coli strain expressing the nuclease gene from the microorganism Serratia marcescens.Reaction conditions: 20 - 37C, optimal buffer: 50 mm Tris-HCl (pH 8.0 at 25C); 100-150 mM NaCl; 1 mM MgCl2.