High grade Mannitol Salt Broth is supplied by Micromaster. It is available in the form of a light yellow to pink coloured homogeneous free flowing powder with a pH in the range of 7.2 to 7.6. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 500g /pack

Mannitol Salt Broth is prepared as suggested by Chapman and is used for the selective isolation of pathogenic Staphylococci. This medium is recommended for the detection and enumeration of coagulase-positive Staphylococci in milk, food, and other specimens. Mannitol Salt Broth is used for the isolation of presumptive pathogenic staphylococci. Pathogenic staphylococci ferment mannitol and produce a yellow coloured medium. The medium contains beef extract and proteose peptone which makes it highly nutritious as they act as the source of essential growth factors and trace nutrients. Many other bacteria except Staphylococci are inhibited by 7.5% sodium chloride. Mannitol is the fermentable carbohydrate source. The differential action of the medium is attributed to D-Mannitol. Staphylococcus aureus ferments mannitol to produce yellow coloured medium. Most coagulase-negative species of Staphylococci and Micrococci do not ferment mannitol and therefore the medium remains red in colour. The colour of the medium is due to the reactivity of phenol red to the pH of the medium; phenol red is red at pH 8.4 and yellow at 6.8. Presumptive Staphylococcus showing yellow coloured medium should be further tested for production of coagulase.