DNA Electrophoresis
DNA electrophoresis is commonly performed using the technique called as Agarose gel electrophoresis - one of the most widely used technique in molecular biology to separate and visualize macromolecules like DNA on an agarose gel under the influence of an electric field. With a plastic comb, wells are dug into the gel. A standard gene ladder is loaded into the first well of the agarose followed by loading the samples in the rest. The gel is dipped into Tris buffer for electrical conduction. When an electric current is applied, negatively charged DNA migrates towards the positive electrode (Anode). A suitable dye is used to visualize the sample run. The separation occurs in the form of bands corresponding to DNA size and shape. An intercalating agent like Ethidium bromide which fluoresces in the presence of UV light is mixed with the sample to visualize DNA bands under A UV transilluminator.
This separation procedure requires a number of accessories, samples and apparatus. These include buffer solutions, DNA ladders, dyes, gels and solidifying agents, electrophoresis apparatus, casting trays, combs, power packs, electrodes, kits etc. Products required in DNA electrophoresis are available from popular brands such as HiMedia, Fisher BioReagents, Bangalore GeNei on Biomall.in.
1 kb Plus DNA ladder (0.3kb kb to 10 kb), 200 wells
₹ 9,000.00
- Brand:
Aura Biotech - Catalog No.:
ABT-123L - Quantity/Unit: 200 wells/Pack
- Availability: In stock
Ethidium Bromide Solution 10 mg/ml, 1 ml
₹ 1,650.00
- Brand:
Aura Biotech - Catalog No.:
ABT-364S - Quantity/Unit: 1 ml/Pack
- Availability: In stock
Ethidium Bromide Solution 10 mg/ml, 5 ml
₹ 4,700.00
- Brand:
Aura Biotech - Catalog No.:
ABT-364M - Quantity/Unit: 5 ml/Pack
- Availability: In stock
Ethidium Bromide Solution 10 mg/ml, 10 ml
₹ 8,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-364L - Quantity/Unit: 10 ml/Pack
- Availability: In stock
Acrylamide/bis acrylamide solution 30% (19: 1), 2500ml
₹ 5,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-369L - Quantity/Unit: 2×500ml/Pack
- Availability: In stock
Acrylamide/bis acrylamide solution 30% (29: 1), 2500ml
₹ 9,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-370L - Quantity/Unit: 2×500ml/Pack
- Availability: In stock
Acrylamide/bis acrylamide solution 30% (36.5: 1), 2500ml
₹ 9,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-371L - Quantity/Unit: 2×500ml/Pack
- Availability: In stock
Acrylamide/Bisacrylamide solution 40% (37.5 :1), 2500ml
₹ 5,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-372L - Quantity/Unit: 2×500ml/Pack
- Availability: In stock
6X DNA Gel Loading Buffer, 1ml X 5
₹ 1,950.00
- Brand:
Aura Biotech - Catalog No.:
ABT-363M - Quantity/Unit: 1ml X 5/Pack
- Availability: In stock
6X DNA Gel Loading Buffer, 1ml 10
₹ 3,400.00
- Brand:
Aura Biotech - Catalog No.:
ABT-363L - Quantity/Unit: 1ml ×10/Pack
- Availability: In stock
3M Potassium acetate Buffer, 250ml
₹ 1,600.00
- Brand:
Aura Biotech - Catalog No.:
ABT-338M - Quantity/Unit: 250ml/Pack
- Availability: In stock
PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free, 11000ml
₹ 7,600.00
- Brand:
Aura Biotech - Catalog No.:
ABT-339L - Quantity/Unit: 1×1000ml/Pack
- Availability: In stock
PBS - Phosphate-Buffered Saline (1X) pH 7.4, RNase-free, 1000ml
₹ 4,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-340L - Quantity/Unit: 1000ml/Pack
- Availability: In stock
2x Laemmli Sample Buffer, 25ml
₹ 1,300.00
- Brand:
Aura Biotech - Catalog No.:
ABT-365L - Quantity/Unit: 2×5ml/Pack
- Availability: In stock
5x Laemmli Sample Buffer, 25ml
₹ 2,350.00
- Brand:
Aura Biotech - Catalog No.:
ABT-366L - Quantity/Unit: 2×5ml/Pack
- Availability: In stock
5X Protein loading buffer(reducing), 25ml
₹ 2,400.00
- Brand:
Aura Biotech - Catalog No.:
ABT-367M - Quantity/Unit: 2×5ml/Pack
- Availability: In stock
5X Protein loading buffer(reducing), 55ml
₹ 5,800.00
- Brand:
Aura Biotech - Catalog No.:
ABT-367L - Quantity/Unit: 5×5ml/Pack
- Availability: In stock
5X Protein loading buffer (non-reducing), 25ml
₹ 2,400.00
- Brand:
Aura Biotech - Catalog No.:
ABT-368M - Quantity/Unit: 2×5ml/Pack
- Availability: In stock
5X Protein loading buffer (non-reducing), 55ml
₹ 5,800.00
- Brand:
Aura Biotech - Catalog No.:
ABT-368L - Quantity/Unit: 5×5ml/Pack
- Availability: In stock
Tris -SDS buffer (pH 8.8) (2.5X), 51000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-375L - Quantity/Unit: 5×1000ml/Pack
- Availability: In stock
Tris -SDS buffer (pH 6.8) (5X)), 51000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-376L - Quantity/Unit: 5×1000ml/Pack
- Availability: In stock
5X Tris-glycine- sds gel running buffer, 51000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-377L - Quantity/Unit: 5×1000ml/Pack
- Availability: In stock
10X Tris-glycine- SDS gel running buffer, 51000ml
₹ 10,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-378L - Quantity/Unit: 5×1000ml/Pack
- Availability: In stock
20X MOPS- SDS Running buffer, 5X1000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-379L - Quantity/Unit: 5X1000ml/Pack
- Availability: In stock
20X MES- SDS Running buffer, 5X1000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-380L - Quantity/Unit: 5X1000ml/Pack
- Availability: In stock
10X Tris acetate-SDS Running buffer, 5X1000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-381L - Quantity/Unit: 5X1000ml/Pack
- Availability: In stock
Tris-Glycine Transfer Buffer (10X), 5X1000ml
₹ 7,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-382L - Quantity/Unit: 5X1000ml/Pack
- Availability: In stock
10X Tris- Glycine buffer, pH 8.3, 5 Litre
₹ 10,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-383L - Quantity/Unit: 5 Litre/Pack
- Availability: In stock