Method Jendrassik & Grof Readytouse liquid stable reagents Simple test procedure : 1.0 ml + 0.02 ml + 0.05 ml Results via factor : 20.2 at 530 nm. 550 nm. Autozyme Bilirubin is a reagent set for determination of Total and Direct Bilirubin based on Jendrassik & Grof method, using Diazotized Sulfanilic Acid with DMSO as an activator for Total Bilirubin Autozyme Bilirubin is a two reagent system using one procedure Autozyme Bilirubin is linear up to 20mg% Autozyme Bilirubin has one step reconstitution Autozyme Bilirubin can be determined in just 5 minutes Autozyme Bilirubin method is relatively free of interference from hemoglobin and other commonly occuring substances in serum or plasma Kit contains: R1: 4x 10 ml R2: 1 x 3 ml