Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins.1,2 The COX component converts arachidonic acid to the hydroperoxy endoperoxide prostaglandin G2 (PGG2; Item No. 17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Item No. 17020). COX-2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions.3 Thus, COX-2 has been the focus of attention for the nonsteroidal anti-inflammatory drug (NSAID) development. Cayman's COX-2 (human recombinant) contains an N-terminal hexahistidine tag and an alanine substituted for arginine at position 508 (COX-2N580A) that prevents glycosylation at position 580 and decrEAses enzyme degradation in cells.4 Technical Information Synonyms Cyclooxygenase 2 Prostaglandin H Synthase 2 Purity 75% (estimated by SDS-PAGE) Source Active recombinant N-terminal His-tagged COX-2N580A mutant purified from insect cells Amino Acids 20-604 MW 67.9 kDa Formulation 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% Tween 20, and 10% glycerol Specific Activity >8,000 U/mg UniProt Accession P35354