Nitric oxide synthase (NOS) is responsible for the biosynthesis of NO from L-arginine. Constitutively expressed NOS is found in brain (nNOS) and endothelial cells (eNOS), wherEAs macrophages can be induced by cytokines or endotoxin to express NOS (iNOS).1 eNOS undergoes several post-translational modifications, including myristolation and palmitoylation, which causes the enzyme to become membrane-associated.2Technical InformationSynonymsecNOSEndothelial Nitric Oxide SynthaseNOS IIIPuritycell lysate 100,000 x g supernatantSourcerecombinant enzyme; isolated from a Baculovirus overexpression system in Sf9 cellsMW135 kDaFormulation50 mM HEPES, pH 7.4, containing 10% glycerol, 5 mM CHAPS, and 100 M DTTUniProt Accession P29473