Fatty acid desaturase 1 (FADS1), also known as 5 desaturase, is a 444-amino acid transmembrane protein encoded by FADS1.1 This product is the N-terminal cytoplasmic domain of FADS1, corresponding to residues 2-121 of the full-length sequence. It catalyzes conversion of dihomo--linolenic acid (Item No. 90230) to arachidonic acid (Item No. 90010) and -3 arachidonic acid (Item No. 90011) to eicosapentaenoic acid (Item Nos. 90110 | 90110.1 | 21908) during the production of -6 and -3 long-chain polyunsaturated fatty acids (LC-PUFAs), respectively. Hepatic FADS1 protein levels and mRNA expression are incrEAsed in mice with high-fat diet-induced obesity and non-alcoholic stEAtohepatitis (NASH).2 Tissue-selective knockdown of fads1 in the liver, adipose tissue, and reticuloendothelial system of adult hyperlipidemic LDL receptor-null mice promotes hepatic inflammation and formation of atherosclerotic plaques and suppresses hepatic lipogenesis.3 IncrEAsed expression of FADS1 positively correlates with disEAse-free survival and overall survival times in patients with esophagEAl squamous cell carcinoma (ESCC).4Technical InformationSynonymsAcyl-CoA (8-3)-Desaturase, 5 Desaturase5D5 DesaturaseEC 1.14.19.FADS1FADS5FADS65 Fatty Acid DesaturaseLinoleoyl-CoA Desaturase (6 Desaturase)-Like 1TU12Purity80% estimated by SDS-PAGESourceRecombinant N-terminal His-tagged FADS1 N-terminal domain purified from E. coliAmino Acids2-121MW15.89 kDaFormulation50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerolUniProt Accession O60427