Recombinant enzyme expressed in E. coli One unit of enzyme produces 1 nmol of nitric oxide per minute at 37C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 0.15 mM NADPH, 4.5 M oxyhemoglobin, 18 M tetrahydrobiopterin, and 180 M DTT.1,2Technical InformationSynonymsInducible Nitric Oxide SynthaseNOS IIPuritycell lysate 100,000 x g supernatantMW130 kDaFormulation50 mM HEPES, pH 7.4, containing 10% glycerol and 8 M BH4Enzyme Commission1.14.13.39UniProt Accession P29477