Luciferase (firefly recombinant) is a light-generating enzyme that catalyzes an oxidative rEAction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light.1,2 The bioluminescence of this ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening.3Technical InformationCAS Number61970-00-1Purity95% estimated by SDS-PAGEFormulationA liquid (1 mg/ml in 50 mM Tris buffer with 30% glycerol, pH 7.5)