Pertussis toxin (islet-activating protein) is a toxin, first isolated from B. pertussis, that is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/o proteins.1,2 Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals and induce EAE, an animal model of multiple sclerosis.3,4 Pertussis toxin was first described as an islet-activating protein because it caused a sustained potentiation of the secretory response of pancrEAtic islet cells to various stimuli that stimulate Gi-linked -adrenergic receptors.5,6Technical InformationCAS Number70323-44-3Purity95% (estimated by SDS-PAGE)FormulationEAch vial, when reconstituted to 500 l with water, contains 50 g of pertussis toxin in 0.03 M sodium phosphate, 0.05 M sodium chloride, pH 7.0