The Plant Direct GenAmp PCR Kit is fast and easy to perform PCR for large sample numbers. The kit provides enough reagents for carrying out 50 reactions. In addition, only a very small sample is also enough for the assay. The protocol involves direct PCR amplification from the plant material without a DNA extraction step. The leaf material is crushed in one buffer, followed by dilution and incubation in a second buffer. The Plant Direct GenAmp PCR Kit represents a considerable simplification of present approaches by eliminating DNA isolation prior to PCR amplification. The primer set should -be stored at 4-degree Celsius for short term storage and at -20-degree Celsius for long term storage.