Peroxisome proliferator-activated receptors (PPARs) are members of the nuclEAr receptor family of ligand activated transcription factors that heterodimerize with retinoic acid like receptor to regulate gene expression and differentiation.1 The PPAR family of nuclEAr hormone receptors consists of three subtypes encoded by separate genes: PPAR, PPAR (also referred to as hNUC1, PPAR, or FAAR), and PPAR. Among them PPAR is highly expressed in tissues displaying a high catabolic rate of fatty acids such as the liver, skeletal muscles, brown fat, hEArt, kidneys, and cells of artherosclerotic lesions.2 Fatty acids, NSAIDs, prostaglandins, leukotriene B4, WY 14643, and hypolipidemic drugs are PPAR ligands.3,4,5 PPAR transcriptionally regulates a variety of genes involved in fatty acid metabolism and oxidation, such as acyl-CoA oxidase, enol-CoA hydratase, medium chain fatty acyl-CoA dehydrogenase, fatty acid transport protein, and cytochrome P450 4A isozymes.6,7 Caymans PPAR LBD (amino acids 170-430 of full length human PPAR) has been expressed and purified from E. coli. The purity was determined using gel electrophoresis followed by coomassie staining and western blot analysis.Technical InformationSynonymsPeroxisome Proliferator-activated Receptor Ligand Binding DomainPurity55%Sourcerecombinant His-tagged protein purified from E. coliAmino Acids170-430 (full length)MW~34 kDa/subunitFormulation50 mM sodium phosphate, pH 7.2, containing 20% glycerol and 100 mM sodium chlorideUniProt Accession Q07869