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PCR Foils and Seals

PCR Foils and Seals

Polymerase chain reaction or PCR is a technique which amplifies or makes multiple copies of a particular sequence of DNA in interest. It initially involves the denaturation of the double-stranded DNA at a temperature of 94 to 96C. This is followed by the annealing or joining of the specifically designed primer at the target site and finally, polymerase action leading to its elongation by the addition of the nucleotides.
There are several variations of PCR including gradient PCR, digital PCR, Quantitative PCR (qPCR), Real Time-PCR, RT-PCR, nested PCR, multiplex PCR and so on. Adhesive foils and seals are films used to cover and seal PCR plates, protecting them from evaporation losses and contamination. These seals can be easily removed leaving no residue and have a broad working temperature range. Some seals are clear to inspect samples, while foils can be pierced with pipette tips.
This molecular biology tool requires a variety of plasticware and other requirements. These encompass adhesive seals, aluminium foils, seal applicators, gas permeable seals, sealing mats etc. These products can be bought online on Biomall, available from renowned brands such as Greiner, Sorenson Bioscience, ABgene.
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