PCR
Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.
In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.
Taq DNA Polymerase-603300051730
- Brand:
Bangalore GeNei - Catalog No.:
603300051730 - Quantity/Unit: 1000 Units/Pack
- Availability: In stock
Taq DNA Polymerase-603400051730
- Brand:
Bangalore GeNei - Catalog No.:
603400051730 - Quantity/Unit: 1000 Units/Pack
- Availability: In stock
GeNei Red Taq DNA Polymerase-603500051730
- Brand:
Bangalore GeNei - Catalog No.:
603500051730 - Quantity/Unit: 1000 Units/Pack
- Availability: In stock
GeNei Red Taq DNA Polymerase-603600051730
- Brand:
Bangalore GeNei - Catalog No.:
603600051730 - Quantity/Unit: 1000 Units/Pack
- Availability: In stock
GeNei Red Taq DNA Polymerase-603700051730
- Brand:
Bangalore GeNei - Catalog No.:
603700051730 - Quantity/Unit: 1000 Units/Pack
- Availability: In stock
Fungal Primer Set - Full (25)-692300251730
- Brand:
Bangalore GeNei - Catalog No.:
692300251730 - Quantity/Unit: 25 No/Pack
- Availability: In stock
XT-5 PCR system 250 U-601800031730
- Brand:
Bangalore GeNei - Catalog No.:
601800031730 - Quantity/Unit: 250 U/Pack
- Availability: In stock
GeNei RAPD Application Teaching Kit-6110000011730
- Brand:
Bangalore GeNei - Catalog No.:
6110000011730 - Quantity/Unit: 5 expts./Kit
- Availability: In stock
GeNei Student PCR Teaching Kit-6104400021730
- Brand:
Bangalore GeNei - Catalog No.:
6104400021730 - Quantity/Unit: 20 expts./Kit
- Availability: In stock
dNTP Mix, 100 mM, 1000 l
- Brand:
Bangalore GeNei - Catalog No.:
652200021730 - Quantity/Unit: 1 EA/Pack
- Availability: In stock
dNTP Mix, 40 mM, 1000 l
- Brand:
Bangalore GeNei - Catalog No.:
652300021730 - Quantity/Unit: 1 EA/Pack
- Availability: In stock
MgCl2 15mM-653500011730
- Brand:
Bangalore GeNei - Catalog No.:
653500011730 - Quantity/Unit: 2 ml/Vial
- Availability: In stock
dNTP Set, 10 mM-651300021730
- Brand:
Bangalore GeNei - Catalog No.:
651300021730 - Quantity/Unit: 20 vials/Set
- Availability: In stock
dATP, 100 mM-650600011730
- Brand:
Bangalore GeNei - Catalog No.:
650600011730 - Quantity/Unit: 100 µl/Set
- Availability: In stock
dGTP, 100 mM-650700011730
- Brand:
Bangalore GeNei - Catalog No.:
650700011730 - Quantity/Unit: 100 µl/Set
- Availability: In stock
dTTP, 100 mM-650800011730
- Brand:
Bangalore GeNei - Catalog No.:
650800011730 - Quantity/Unit: 100 µl/Set
- Availability: In stock
dCTP, 100 mM-650900011730
- Brand:
Bangalore GeNei - Catalog No.:
650900011730 - Quantity/Unit: 100 µl/Set
- Availability: In stock
Taq Buffer F -653400011730
- Brand:
Bangalore GeNei - Catalog No.:
653400011730 - Quantity/Unit: 1 ml/Vial
- Availability: In stock
XT-5 PCR system 1000 U-601800051730
- Brand:
Bangalore GeNei - Catalog No.:
601800051730 - Quantity/Unit: 1000 U/Pack
- Availability: In stock
XT-20 PCR system 250 U-601900031730
- Brand:
Bangalore GeNei - Catalog No.:
601900031730 - Quantity/Unit: 250 U/Pack
- Availability: In stock
XT-20 PCR system 1000 U-601900051730
- Brand:
Bangalore GeNei - Catalog No.:
601900051730 - Quantity/Unit: 1000 U/Pack
- Availability: In stock
GeNei Long PCR 20 kb Ampli Kit-667600011730
- Brand:
Bangalore GeNei - Catalog No.:
667600011730 - Quantity/Unit: 50 reactions/Pack
- Availability: In stock
Taq DNA Polymerase-602700061730
- Brand:
Bangalore GeNei - Catalog No.:
602700061730 - Quantity/Unit: 5000 Units/Pack
- Availability: In stock
Taq DNA Polymerase-602900051730
- Brand:
Bangalore GeNei - Catalog No.:
602900051730 - Quantity/Unit: 1000 Units/Pack
- Availability: In stock
M-MuLV Reverse Transcriptase-601300061730
- Brand:
Bangalore GeNei - Catalog No.:
601300061730 - Quantity/Unit: 5000Units/Pack
- Availability: In stock
GeNei M-MuLV RT-PCR Kit-667400011730
- Brand:
Bangalore GeNei - Catalog No.:
667400011730 - Quantity/Unit: 20 reactions/Pack
- Availability: In stock
GeNei One Step M-MuLV RT-PCR Kit-661700021730
- Brand:
Bangalore GeNei - Catalog No.:
661700021730 - Quantity/Unit: 25 reactions/Pack
- Availability: In stock
GeNei One Step M-MuLV RT-PCR Kit-661700051730
- Brand:
Bangalore GeNei - Catalog No.:
661700051730 - Quantity/Unit: 100 reactions/Pack
- Availability: In stock