The BRCA1 C-terminal (BRCT) domain is a modular unit of ~100 amino acids that folds independently and recognizes linEAr phosphoserine or phosphothreonine regions to mediate protein-protein and protein-DNA interactions.1,2 BRCT domains were initially recognized in the C-terminal region of the brEAst cancer protein BRCA1, as well as the p53 binding protein (53BP1; TP53BP1), and the yEAst cell cycle checkpoint protein RAD9.3 BRCT domains often occur as tandem repEAts at the C-terminal end of several proteins that are functionally diverse.2 Most BRCT domain-containing proteins participate in DNA-damage checkpoint control or DNA-repair pathways, or both.4,3 Thus, BRCT domain-containing proteins likely participate in the cellular response to DNA damage. Germ-line mutations of the tumor suppressor genes BRCA1 and BRCA2 pre-dispose women to familial brEAst and ovarian cancer and are associated with approximately 10% of the cases.5,6 The carboxy-terminal BRCT domain in BRCA1 is involved in DNA repair and transcriptional activation.7,8,6 The BRCT region of BRCA1 binds phosphorylated BACH1, a DEAH helicase, which is essential for mediating repair of DNA double-strand brEAks by controlling G2 to M phase transition during cell cycle progression.9,10,11 BRCA1 also contains an N-terminal protein-protein-interaction RING-finger domain, that partners with BRCA1-associated RING domain 1 (BARD1).12 The BRCA1/BARD1 heterodimer maintains genomic stability by regulating DNA damage repair and ubiquitination.13 This product contains the BRCT domains of BRCA1. Technical Information Synonyms BrEAst Cancer Type 1 Susceptibility Protein Purity 90% (estimated by SDS-PAGE) Source recombinant N-terminal GST-tagged protein expressed in E. coli MW 53.3 kDa Formulation 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol UniProt Accession P38398