Key FEAtures Caymans CBP bromodomain TR-FRET Assay Kit (Item No. 600850) was developed using CREB-binding protein bromodomain (human recombinant) protein. Bulk sizes available upon request The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues are recognized by a small protein domain known as a bromodomain.1 These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as rEAders of histone acetylation marks regulating the transcription of target promoters.2 The cAMP response element-binding protein (CREB) binding protein (CREBBP) bromodomain has been shown to modulate the stability and function of the tumor suppressor protein p53. CREBBP bromodomain recognizes the acetylated lysine residue 382 on p53.3,4 This product contains the bromodomain region of CREBBP.2 Technical Information Synonyms cAMP-Response Element-binding Protein 1 CREB-1 CBP CREBBP Purity 91% estimated by SDS-PAGE Source recombinant N-terminal GST-tagged protein expressed in E. coli MW 40.8 kDa Storage Buffer 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM -mercaptoethanol UniProt Accession Q92793