Spoligotyping is a PCR- based Reverse Line Hybridization method to simultaneously detect and type Mycobacterium tuberculosis complex bacteria in clinical samples, exploiting polymorphism in the "Direct Repeat" (DR) region, which is uniquely present in Mycobacterium tuberculosis complex bacteria which includes Mycobacterium tuberculosis, M. africanum, M. bovis, M. canettii, M. microti, M. caprae, and M. pinnipedii. The presence or absence of the strains can be detected by hybridization of PCR amplified sample to a set of immobilized oligonucleotides on the membranes.
Foam Cushions (PC200) :
Foam Cushions are intended for use with, Mini-blotter which enables you to perform multiple antibody or probe incubation on a single membrane, using microliter volumes of the reagent. Typical applications include monoclonal screening on western blots, animal and human screening, and DNA hybridization. Sample processing is streamlined through the use of washing manifold, which enables washing of all sample lanes, simultaneously. Reactions can be detected by all conventional methods, including enzyme conjugates, chemiluminescence, and radiolabels.