Hexa Pseudo 3-HX013-1PK is 6-in-one multi disc, a hexa disc, supplied by HiMedia. It contains an inert flat circular hexa ring with 6 equidistant arms on the outer periphery, each arm having a 6mm disc. Each disc is saturated with a specific antibiotic, that help in antibiotic susceptibility testing (AST) of Pseudomonas bacteria. Each antibiotic disc is about 24mm apart from the adjacent disc, and is labeled with the concentration and symbol of the respective antibiotic.
The composition of antibiotics on the discs are as:
Amikacin 30mcg
Carbenicillin 100mcg
Ciprofloxacin 5mcg
Gentamicin 10mcg
Tobramycin 10mcg
Mezlocillin 75 mcg
The Hexa Pseudo 3-HX013 rings are deposited aseptically in the center of the inoculated Mueller Hinton agar plate using sterile forceps. Immediately the plates are incubated at 352C for 16-18 hours. For fastidious organisms, incubate at appropriate temperature and time. The diameter of the zones of complete inhibition are measured to the nearest mm.
These multidiscs are convenient and economic for conducting AST. They give the priviledge to study large number of antibiotics at one time.
The Hexa Pseudo 3-HX013 discs should be stored at -20C under dry conditions, along with the dessicator provided in indivisual pack.