Key FEAturesCaymans JMJD2A Tudor Domains TR-FRET Assay Kit (Item No. 700960) was developed using JMJD2A tudor domains (human recombinant) protein.Bulk sizes available upon requestTudor domains are small protein structural motifs of about ~50 amino acids related to the royal family of methyl rEAders, which also includes chromo, MBT, PWWP, and Agenet-like domains.1,2 Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are invloved in RNA metabolism, germ cell development, transposon silencing, DNA damage response, histone modification, and chromatin remodeling.3 The tudor domains recognize symmetric methylated arginine or methylated lysine residues.4,5,6,7 JMJD2A catalyzes the demethylation of trimethylated histone H3 at lysine residues 9 and 36 (H3K9me3 and H3K36me3).8 However, the tudor domain of this protein has been shown to bind histone H3K4me, H3K9me3, and H3K20me2/3.4 Like other JmjC protein hydroxylase family members, JMJD2A is an -ketoglutarate-dependent Fe (II) oxygenase.8 This product contains the tandem tudor domains of JMJD2A.Technical InformationSynonymsJHDM3AJumonji Domain Containing 2AKDM4APurity90% estimated by SDS-PAGESourcerecombinant N-terminal GST-tagged protein expressed in E. coliMW42.2 kDaFormulation50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol