Key FEAturesCaymans L3MBTL1 MBT Domains TR-FRET Assay Kit (Item No. 601030) was developed using L3MBTL1 MBT domains (human recombinant; GST-tagged) protein.Bulk sizes available upon request"The malignant brain tumor (MBT) domain is structurally related to chromatin binding domains, such as chromodomains, tudor domains, and PWWP-domains.1,2 MBT domain-containing proteins have a variable number of MBT repEAts. The MBT domains recognize methylated lysines on histone tails with varying degree of specificity for the various methyl marks.1 L3MBTL1, a human homolog of the Drosophila lethal(3)MBT protein, is a member of the polycomb group (PcG) of proteins that functions as a transcriptional repressor.3 L3MBTL1 contains three MBT repEAt domains which collectively bind to either histone H3 or H4.1,4 The second and third MBT domains were found to bind preferentially to mono- and dimethylated lysines of histone H3 at lysine 4 (H3K4me1) and histone H4 at lysine 20 (H4K20me2).4,5 Recognition of methyl-lysine marks by MBT domains lEAds to chromatin compaction and a repressed transcriptional state.4,6 Further, L3MBTL1 has a tumor suppressor function and is thought to play a role in maintaining genomic stability and DNA replication.7,8 This protein product contains the MBT repEAt region of L3MBTL1."Technical InformationSynonymsH-l(3)MBTLethal(3)Malignant Brain Tumor-like Protein 1L(3)MBT-likePurity90% estimated by SDS-PAGESourcerecombinant N-terminal GST-tagged protein expressed in E. coliMW65.5 kDaFormulation50 mM Tris, pH 8.0, containing 150 mM sodium chloride and 20% glycerol