MBT072-1000U is referred as M-MuLV Reverse Transcriptase, under HiMedia catalogue. M-MuLV Reverse Transcriptase (Molony Murine Leukemia Virus Reverse Transcriptase) is an RNA-dependent DNA polymerase that requires a DNA primer and an RNA template to synthesize a complementary DNA strand. It has a weaker intrinsic RNase H activity than AMV RTase (Avian Myeloblastosis Virus Reverse Transcriptase). The weaker RNaseH activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs
Specifications:
Concentration : 200 units/l
Kit contains: M-MuLV Reverse Transcriptase (RNase H-),RT Buffer for MMuLV and uLV DS0279 30 L 130 L
10X Solution
Applications:1) First strand cDNA synthesis2) Preparation of cDNA Probe 3)For RT-PCR & Real time RT-PCR
Storage Conditions: Store at -20C
Pack size :1000U