Spoligotyping is a PCR- based Reverse Line Hybridization method to simultaneously detect and type Mycobacterium tuberculosis complex bacteria in clinical samples, exploiting polymorphism in the "Direct Repeat" (DR) region, which is uniquely present in Mycobacterium tuberculosis complex bacteria which includes Mycobacterium tuberculosis, M. africanum, M. bovis, M. canettii, M. microti, M. caprae, and M. pinnipedii. The presence or absence of the strains can be detected by hybridization of PCR amplified sample to a set of immobilized oligonucleotides on the membranes.
Mini-blotter (MN45) :
Mini-blotter, enable you to perform multiple antibody or probe incubations on a single membrane, using microlitre volumes of reagent. Typical applications include monoclonal antibody screening on western blots, animal and human screening, and DNA probehybridizations. Sample processing is streamlined through the use of the washing manifold, which enables washing of all the sample lanes, simultaneously. Reactions can be detected by all conventional methods, including enzyme conjugates, chemiluminescence and radiolabels.